elisa kits  (R&D Systems)

Journal: Communications Biology

Article Title: Tick saliva reprograms macrophages into immunosuppressive hubs that regulate T-cell immunity in Rhipicephalus microplus infestation

doi: 10.1038/s42003-026-09981-5

Figure Lengend Snippet: A Representative histograms showing the expression intensity of MHC class II on macrophages stimulated with LPS and IFN-γ in the presence or absence of Rm-saliva. B Summary of the MFI of MHC class II on macrophages. C Summary of the proportions of MHC class II⁺ on macrophages. D Representative flow cytometry plots showing co-expression of MHC class II and CD80. E Summary of the frequencies of MHC class II⁺CD80⁺ macrophages. F Representative flow cytometry plots showing co-expression of MHC class II and CD86. G Summary of the frequencies of MHC class II⁺CD86⁺ macrophages. Concentrations of CCL2 ( H ), CCL3 ( I ), CCL4 ( J ), CCL5 ( K ), CCL8 ( L ), CXCL9 (M) in culture supernatants of macrophages stimulated with LPS and IFN-γ in the presence or absence of Rm-saliva. Chemokine concentrations were generally low under unstimulated conditions, with some values falling below the assay’s detection limit. Data represent paired samples from nine individual cattle ( n = 9). * P < 0.05 (Friedman test followed by the Wilcoxon signed-rank test).

Article Snippet: The concentrations of chemokines in macrophage culture supernatants were determined using the BOVINE CCL2, CCL3, CCL4, CCL5, CCL8, and CXCL9 DO-IT-YOURSELF ELISA Kits (Kingfisher Biotech).

Techniques: Expressing, Flow Cytometry

Journal: bioRxiv

Article Title: Translational toolkit for reproducible, cross-study profiling of human ageing hallmarks in human blood and tissue

doi: 10.64898/2026.04.20.719545

Figure Lengend Snippet: A. Schematic overview of a novel 32-color spectral flow cytometry panel for profiling circulating immune populations and intracellular senescence markers p16 and p21. B. PCA analysis of young and aged peripheral blood mononuclear cells (PBMCs), integrating all identified immune cell subsets. C. IMMAge score calculated based on PBMC subsets for young and aged participants in young (n=8) and aged (n=12) participants. D. CD4+ CD57+CD28- and E. CD8+ CD57+CD28-senescent cells expressed as a percentage of total CD4+ or CD8 T cells in young (n=8) and aged (n=12) participants. Intracellular p21 expression in F. Naïve and G. EMRA CD8+ T cells from (n=9) young and aged (n=12) participants. H. Intracellular p16 expression assessed as median fluorescent intensity in Memory B cells (CD19+ CD38− CD24+) from (n=9) young and aged (n=12) participants. Expression of CD95 in I. CD4+ and J. CD8+ T cells between in young (n=8) and aged (n=12) participants. K. Schematic overview of cryosection and whole-tissue approaches for detecting cellular senescence using β-galactosidase staining (SA-beta gal) in human adipose tissue. Representative images of positive β-galactosidase staining (blue) of senescent cells in L. 20 µM human adipose tissue cryosections and M. whole human adipose tissue following 24h incubation. Expression of SASP markers N. GDF15, O. CXCL-9, P. TNF alpha, and Q. IL-6 in plasma samples from young (n=5) and aged (n=12) participants, determined by ELISA. Data are mean ± S.E.M. Statistical significance was assessed (C-F, H-J) unpaired t-tests or (G, N-Q) Mann Whitney U.

Article Snippet: Serum and EDTA plasma concentration of CXCL-9, GDF-15, IL-6 and TNFα, were measured using commercially available ELISA kits (CXCL9; DY392-05, GDF15; DY957, IL-6; DY206, TNF; DY210, R&D Systems, MN, USA) following the manufacturer’s protocol.

Techniques: Flow Cytometry, Expressing, Staining, Incubation, Clinical Proteomics, Enzyme-linked Immunosorbent Assay, MANN-WHITNEY